Journal: Journal of Cancer

Article Title: Ribonucleotide Reductase Inhibitor 3-AP Induces Oncogenic Virus Infected Cell Death and Represses Tumor Growth

doi: 10.7150/jca.27437

Figure Lengend Snippet: 3-AP treatment represses KSHV-infected endothelial cell proliferation and induces DNA damage. ( A ) TIVE-LTC and TIVE were incubated with indicated concentrations of 3-AP for 72 h, then cell proliferation was measured using the WST-1 assays. ( B-D ) TIVE-LTC were incubated with indicated concentrations of 3-AP for 48 h and cell viability was measured by using flow cytometry as described in the Methods. Protein expression and DNA damage were measured by using immunoblots and CometAssay, respectively. Error bars represent the S.D. for 3 independent experiments. *= p<0.05; **= p<0.01.

Article Snippet: The tail moment was calculated from 100 cells collected per single measurement by utilizing specialized comet software included in the Automated CometAssay System (Loats Associates Inc).

Techniques: Infection, Incubation, Flow Cytometry, Expressing, Western Blot